Xirmada ogaanshaha ELISA ee ka hortagga caabuqa ee wiyisha ee Bovine

Qaabka No:VD105E
Abbr.:IBRV Ab Elisa
Tusaale: serum

Sharaxaad

Tilmaamid

BVDV Ab Elisa

Isticmaalka la damacsan yahay: CaabuqaFayraska Rhinotracheitis ee BovineQalabka ogaanshaha ELISA ee ka-hortagga unugga ayaa loo isticmaalaago'aamin rhinotracheitis faafadifaacayaasha fayraska ee serum bovine in vitro.

Tijaabada: serum

Agabka la bixiyay:

Infectious Bovine Rhinotracheitis Virus Antibody ELISA Detection Kit

Habka Imtixaanka & Fasiraadda

TALLAABADA 1 Habka Imtixaanka

1.1 Diyaarinta Xalka Dhaq

Wfiirsashada orking wdareere dambas ah:Ka hor inta aan la isticmaalin, xalinta dhaqidda waa in lagu soo celiyaa heerkulka qolka (20-25 digrii) oo si siman loo ruxayaa si ay u noqoto mid labbis ah. Dabadeed ku qas 20 jeer xalalka dhaqidda ee xoogga leh oo leh biyo nadiif ah (tusaale ahaan, 5ml xal dhaqid oo xoog leh ayaa lagu daraa 95ml biyo nadiif ah), si fiican isku qas, dareeraha dhaqidda ee la qasi karo waxaa lagu kaydin karaa 2-8 digrii 7 maalmood.

1.2 Tusaalaha Diyaarinta

① Dhiigga xoolaha oo dhan ayaa la qaaday, waxaa la saaray 4000rpm 10 daqiiqo, waxaana la ururiyey maadada sare. Serum sidoo kale si dabiici ah ayaa loo xinjiraysan karaa iyada oo aan la helin dhiig-baxa.

② Ku qas serum si loo tijaabiyo 50 jeer saxanka milanka serum (tusaale: ku dar 3ul serum si loo tijaabiyo 147ul muunad dareere ah).

Fiiro gaar ah: Kontaroolada xun iyo kuwa togan looma baahna in la qaso

1.3 Habka Imtixaanka

① Qaado calaamadda enzyme Microplate (sida ku cad tirada shaybaarrada, waa la kala diri karaa oo la isticmaali karaa dhowr jeer), ku dar 100ul ee serum-ka la qasi jiray calaamadda enzyme Microplate, dhig laba ceel mid kasta si loo xakameeyo taban iyo xakamaynta togan, oo ku dar 100ul si fiican u siday u kala horreeyaan. Si tartiib ah u rux muunada ceelka, adigoo isticmaalaya filimka saxanka daboolaya si aad u daboosho saxanka, oo ku dabool 37 darajo 30 daqiiqo.

② Fur filimka saxanka, ka rux xalka saxanka si fiican, ku dar 300µl oo dareere dhaqid ah oo shaqaynaya ceel kasta, ka dibna tuur dareeraha ceelka. Ku celi tillaabooyinka kore 5 jeer, oo ugu dambeyntii ku qallaji warqad nadiif ah oo nuugta.

③ Isku xidhka enzymka la badhxay: Isku qas enzyme conjugate (80×) 80 jeer (tusaale: 10ul enzyme conjugate concentrate + 790ul sample diluent, qaddarka gaarka ah ee loo baahan yahay waxaa loo xisaabiyaa iyadoo loo eegayo tirada tijaabada tijaabada), si fiican isku qas oo dhinac dhig.

Ku dar 100µl of Enzyme Conjugate ceel kasta, dabool saxanka, oo ku dheji 37 darajo 30 daqiiqo.

⑤ Dhaqidda saxanka enzyme, hawlgalka gaarka ah wuxuu la mid yahay kan sare ②.

⑥Ku dar 100µl xal substrate ceel kasta, dabool saxanka oo ku dabool 10 daqiiqo meel 37 digrii ka fog iftiinka.

TALLAABADA 2 Fasiraadda Natiijooyinka

Qiimaha OD450 ee ceel kasta waxaa lagu akhriyay 96-well microplate akhristaha.

2.1 Xaqiijinta Celceliska qiimaha OD450nm ee kantaroolka taban wuxuu ahaa <0.25 iyo celceliska OD450nm ee xakamaynta togan ayaa ka weyn ama la mid ah 0.6, natiijooyinka tijaabada ayaa sax ahaa. Haddii kale, baaritaanka waa in lagu celiyaa.

2.2 Habka Xisaabinta

Muunad kasta, u xisaabi S/P sida soo socota adoo isticmaalaya muunada la saxay iyo qiimayaasha xakamaynta:

product-554-49

2.3 Fasiraadda Natiijooyinka

Marka S/P <0.2, waxaa lagu qiimeeyaa mid taban.

Marka S/P> 0.3, waxaa lagu qiimeeyaa mid togan.

Marka 0.2 uu ka yar yahay ama le'eg yahay S/P In ka yar ama le'eg yahay 0.3, waxaa loo qiimeeyaa inay tahay shaki, oo soo jeedi inaad ku celiso muunada oo tijaabi hal usbuuc ka dib.

Hot Tags: Xirmada ogaanshaha elisa ee fayraska caabuqa ee bovine rhinotracheitis fayraska ka hortaga fayraska elisa alaab-qeybiyeyaasha, warshadeeyayaasha, warshad

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